Fig. 2
In vivo (left panel) representative zymographic activities of SOD, catalase and GPx activity in tumor and corresponding surrounding tissue (in-vivo). SOD1: a 100 µg protein loaded, lane distribution: 1—rat liver; 2, 3, 4—surrounding; 5, 6, 7—tumor; b 100 µg protein loaded, lane distribution: 1, 2, 3—surrounding; 4, 5, 6—tumor; 8—human RBC; 9—rat liver. c Lane 1, 2, 3, 4—surrounding tissue; 6, 7, 8, 9—tumor tissue. GPx-d: 100 µg protein loaded—lane distribution: 1, 2, 3, 4—surrounding; 5—plasma; 6, 7, 8, 9, 10—tumor. Catalase: e 100 µg protein loaded—lane distribution: 1, 3, 5—surrounding; 2, 4, 6, 7, 8, 9, 10—tumor. f 100 µg protein loaded—lane distribution: 1, 2, 3—surrounding; 4 to 6—tumor; 8—RBC; 9—rat liver. Densitometry results are mean ± SE (n = 17–23 in different groups). Data of tumor group is compared to the surrounding group (Student ‘t’ test). Levels of significances (p values) are mentioned in the figure. In vitro SOD, catalase and GPx activity modification by redox change with H2O2 and β-ME (in-vitro, right panel). g SOD1: 1, 2—control; 3—0.5 M H2O2; 4—0.1 M H2O2; 5—100 µM BM; 6—10 µM BM; 7—1 µM BM. h SOD1: 125 μg of human RBC protein loaded, Lane: 1, 2—control; 3—100 mM H2O2; 4—1 M H2O2; 5—1 M H2O2 + 1 nM BM; 6—1 M H2O2 + 0.1 μM BM; 7—1 M H2O2 + 1 μM BM; 8—1 M H2O2 + 10 μM BM. i SOD1: 1, 2—control; 3—0.1 M H2O2; 4—0.01 M H2O2; 5—10 μM BM; 6—1 μM BM; 7—0.1 M H2O2 + 100 μM BM. j Catalase: 1, 2—control; 3—500 mM H2O2; 4—1 M H2O–; 5—0.1 μM BME + 1 M H2O2; 6—1 μM BME + 1 M H2O2; 7—10 μM BME + 1 M H2O2. k GPx: 1, 2, 3—control; 4—100 μM H2O2; 5—500 mM H2O2; 6—1 M H2O2; 7—1 M H2O2 + 1 μM β-ME; 8—1 M H2O2 + 10 μM β-ME; 9—1 M H2O2 + 100 μM β-ME; 10—1 M H2O2 + 1 mM β-ME