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Fig. 4 | Cancer Cell International

Fig. 4

From: A novel positive feedback loop of linc02042 and c-Myc mediated by YBX1 promotes tumorigenesis and metastasis in esophageal squamous cell carcinoma

Fig. 4

Linc02042 interacts with YBX1. a RPISeq database predicting the interaction probability between linc02042 and YBX1. Both RF and SVM classifier greater than 0.5 mean positive. b RNA pull-down coupled Western blotting analysis in ESCC cells. c RIP assays in ESCC cells with IgG and YBX1 antibodies, followed by qRT-PCR analysis of linc02042 expression. d Western blotting analysis of YBX1 protein expression in linc02042-depleted ESCC cells. e RIP assays in ESCC cells with IgG and YBX1 antibodies in linc02042-depleted ESCC cells. f qRT-PCR analysis of c-Myc expression in linc02042-overexpressed cells treated with YBX1 siRNA. g The assessment of cell viability and invasion respectively detected by CCK-8 and Transwell assays in linc02042-overexpressing cells transfected with YBX1 siRNA. **p < 0.01, ***p < 0.001

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