Fig. 2

CRART16 is upregulated in Caco-2 CR. a Scatter plots show the difference in the expression of lncRNAs between Caco-2 and Caco-2 CR cells. Red dots above the red line indicate significantly upregulated lncRNAs, whereas green dots below the green line indicate significantly downregulated lncRNAs. b The expression of the CRART16 was detected in a panel of CRC cell lines (Caco-2, Caco-2 CR, HCT116, SW620 and HCT8) by qRT-PCR. GAPDH was used as an internal control. All experiments were repeated three times, and data are presented as mean ± SD. c The transfection efficiency and morphology of Caco-2-CRART16 and Caco-2-NC cells were viewed by bright field and fluorescence microscopy. Scale bar = 100 μm. d Transfection efficiency of the lncRNA CRART16 was detected in Caco-2-CRART16 and Caco-2-NC cells by qRT-PCR. GAPDH was used as an internal control. All experiments were repeated three times, and data are presented as mean ± SD. **P < 0.01 by Student’s t test