Fig. 5

CRART16 modulates ERBB3 expression in a miR-371a-5p-dependent manner. a The expression of ERBB3, ATP8B1, KAT6A, FKTN, UCHL5, NUF2 and RPS6KA3 was detected by qRT-PCR after lncRNA CRART16 overexpression. All experiments were repeated three times, and data are presented as mean ± SD. **P < 0.01 by two-way ANOVA. b Schematic diagram of the predicted binding sites between ERBB3 and miR-371a-5p. c Dual-luciferase reporter assays in 293T cells. The relative luciferase activity was measured after cotransfection with miR-371a-5p mimics or miR-NC and either the pmiR-RB-Report™-ERBB3 3′ UTR-WT vector or the empty vector. All experiments were repeated three times, and data are presented as mean ± SD. **P < 0.01 by Student’s t test. d The expression of ERBB3 was detected in Caco-2-CRART16 cells after miR-371a-5p knockdown. All experiments were repeated three times, and data are presented as mean ± SD. *P < 0.05, **P < 0.01 by Student’s t test. e The percentage of ERBB3-positive cells and the MFI were determined by GALLIOUS flow cytometry in Caco-2-CRART16 cells after miR-371a-5p knockdown. All experiments were repeated three times, and data are presented as mean ± SD. *P < 0.05, **P < 0.01 by Student’s t test