Fig. 1

Downregulation of ZFPM2-AS1 suppressed malignant behaviors of SCLC cells. a qRT-PCR results of ZFPM2-AS1 level in SCLC cell lines and controls (16-HBE). b qRT-PCR results of ZFPM2-AS1 level following treating DMS-53 and SHP-77 cells with shRNAs targeting ZFPM2-AS1. c, d Colony formation and EdU analyses of DMS-53 and SHP-77 cell proliferation in response to ZFPM2-AS1 knockdown. In EdU assay, EdU-positive cells (stained in red) are cells that were proliferating, and DAPI stained cells (stained in blue) were total cells. e CCK-8 evaluated DMS-53 and SHP-77 cell viability when downregulating ZFPM2-AS1. f Transwell assay detected DMS-53 and SHP-77 cell invasion and migration after silencing ZFPM2-AS1. g Wound healing assay assessed DMS-53 and SHP-77 cell migration upon ZFPM2-AS1 silencing. *P < 0.05, **P < 0.01