Fig. 5

ZFPM2-AS1 contributed to SCLC proliferation, invasion, migration and tumor growth via upregulating TRAF4. a TRAF4 mRNA and protein expressions in SHP-77 cells were measured by qRT-PCR and western blot. b, c Colony formation and EdU analysis of SHP-77 cell proliferation following indicated transfection. In EdU assay, EdU-positive cells (stained in red) are cells that were proliferating, and DAPI stained cells (stained in blue) were total cells. d Transfected SHP-77 cell viability was analyzed by CCK-8. e Transwell assay of SHP-77 cell invasion and migration under indicated treatment conditions. f Wound healing assays of SHP-77 cell migration after indicated transfections. *P < 0.05, **P < 0.01