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Fig. 3 | Cancer Cell International

Fig. 3

From: MYC-regulated lncRNA NEAT1 promotes B cell proliferation and lymphomagenesis via the miR-34b-5p-GLI1 pathway in diffuse large B-cell lymphoma

Fig. 3

Overexpression of miR-34b-5p attenuated cell proliferation and accelerated cell apoptosis in DLBCL in vitro. a The miR-34b-5p levels were measured by RT-qPCR after miR-34b-5p mimic or mimic NC transfection into OCI-Ly1 and SUDHL-4 cell lines. **P < 0.01 vs. mimic NC, T-test. b Cell viability was detected by MTT assay in OCI-Ly1 and SUDHL-4 cell lines transfected with miR-34b-5p mimic or mimic NC. *P < 0.05, **P < 0.01 vs. mimic NC, T-test. c Colony formation assays were performed in OCI-Ly1 and SUDHL-4 cell lines transfected with miR-34b-5p mimic or mimic NC. A representative image (left) is shown, and the total number of colonies per plate were counted (right). **P < 0.01 vs. mimic NC, T-test. d Flow cytometry analysis of apoptotic cells by annexin-V/PI staining following miR-34b-5p mimic or mimic NC in OCI-Ly1 and SUDHL-4 cell lines. A representative image of FACS staining is shown on the left, and the statistical data are shown on the right. *P < 0.05 and **P < 0.01 vs. mimic NC, T-test. e TUNEL staining in miR-34b-5p mimic or mimic NC. A representative image of TUNEL staining (left) and the statistical data (right) are shown. **P < 0.01 vs. mimic NC, T-test. f The mRNA level of GLI1 was assessed by RT-qPCR in OCI-Ly1 and SUDHL-4 lines transfected with miR-34b-5p mimic or mimic NC. *P < 0.05 vs. mimic NC, T-test. g Immunoblotting and quantification of GLI1, Cyclin D1, CDK4, p27 (and β-actin as loading control) in OCI-Ly1 and SUDHL-4 cells transfected with miR-34b-5p mimic or mimic NC. *P < 0.05, **P < 0.01, vs. mimic NC. T-test

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