Fig. 4

Overexpression of lncRNA NEAT1 reverses the suppression of cell proliferation by knockdown of GLI1. a RT-qPCR to determine GLI1 expression in GLI1 knockdown cells (shGLI1) or negative control cells (shNC). **P < 0.01 vs. shNC, T-test. b RT-qPCR to determine NEAT1 expression in NEAT1-overexpressing cells (NEAT1) or negative control cells (Vector). **P < 0.01 vs. Vector, T-test. c RT-qPCR for GLI1 expression in GLI1 knockdown cells (shGLI1), NEAT1 overexpression cells (NEAT1), GLI1 knockdown plus NEAT1 overexpression cells (shGLI1 + NEAT1) and negative control cells (NC). *P < 0.05, **P < 0.01, T-test. d Immunoblotting for GLI1, Cyclin D1, CDK4 and p27 (and β-actin as loading control) in the cell lines described in c. The quantification of each protein is shown. *P < 0.05, **P < 0.01, T-test