Fig. 5

LncRNA NEAT1 acted as ceRNA, sponging miR-34b-5p to regulate GLI1 expression. a Diagram showing that StarBase predicts the interaction between lncRNA NEAT1 and miR-34b-5p. b The effect of wt-NEAT1 or mut-NEAT1 on miR-34b-5p expression detected by dual-luciferase reporter assay. *P < 0.05, **P < 0.01, vs. Control, T-test. c Interaction between wt-NEAT1 or mut-NEAT1 and miR-34b-5p was detected by RIP. **P < 0.01, vs. MS2bs-Rluc or MS2bs-NEAT1 Mt, T-test. d TargetScan predicts the binding region between miR-34b-5p and the GLI1 3′-UTR. e Dual-luciferase reporter assay was performed to analyse the influence of miR-34b-5p on wt-GLI1 3′-UTR or mut-GLI1 3′-UTR. *P < 0.05, **P < 0.01, vs. Control, T-test. f Interaction between miR-34b-5p and GLI1 3′-UTR was detected by RIP. **P < 0.01, vs. MS2bs-Rluc or mut-GLI1 3′-UTR Mt, T-test