Fig. 6

MYC modulated DLBCL proliferation through regulating NEAT1 transcription by binding to the promoter. a RT-qPCR to determine MYC expression in MYC overexpression cells (MYC) or negative control cells (Vector). **P < 0.01, vs. Vector, T-test. b MTT assay to determine cell proliferation in MYC-overexpressing cells (MYC) or negative control cells (Vector). *P < 0.05, **P < 0.01, vs. Vector, T-test. c Colony formation assay was performed in MYC-overexpressing cells (MYC) or negative control cells (Vector). A representative image of the colony formation assay in OCI-Ly1 and SUDHL-4 cells is shown (left), and the total number of colonies per plate was counted (right). *P < 0.05, **P < 0.01, vs. Vector, T-test. d Flow cytometry analysis of apoptotic cells by annexin-V/PI staining following MYC overexpression in OCI-Ly1 and SUDHL-4 cell lines. A representative image of FACS staining is shown on the left, and the statistical data are shown on the right. **P < 0.01 vs. Vector, T-test. e TUNEL staining in MYC-overexpressing cells. A representative image of TUNEL staining (left) and statistical data (right) are shown. **P < 0.01 vs. Vector, T-test. f RT-qPCR to determine NEAT1 expression in MYC-overexpressing cells (MYC) or negative control cells (Vector). *P < 0.05, **P < 0.01, vs. Vector, T-test. g Known or potential binding sites of MYC on the promoter of NEAT1 were examined by JASPAR. h CHIP assay was performed to detect the interaction of MYC on BS1 or BS2. ***P < 0.001, vs. IgG, T-test. i Western blot to determine MYC levels (left panel) and ChIP assay to determine enrichment of MYC on the promoter of NEAT1 (right panel) were performed in MYC knockdown cell lines (shMYC-1, shMYC-2) and negative control cell lines (shNC); *P < 0.05, **P < 0.01, vs. shNC, T-test