Skip to main content
Fig. 5 | Cancer Cell International

Fig. 5

From: MiR-33a functions as a tumor suppressor in triple-negative breast cancer by targeting EZH2

Fig. 5

The knockdown of EZH2 suppresses the malignant behaviors in TNBC cells. a The expression of EZH2 in TNBC cell lines and normal breast epithelial cell was measured by qRT-PCR assay. **P < 0.01, ***P < 0.001 compared with MCF10A cells. b The expression of EZH2 in TNBC cell lines was significantly downregulated after transfection with EZH2 siRNA. c, d MDA-MB-231 and BT-549 cells were transfected with NC or EZH2 siRNA for 24 h and cell proliferation was determined by an EdU cell proliferation kit. EdU positive cells and images were captured. e, f The effect of EZH2 on cell proliferation rate was determined by CCK-8 assay. g Decreased miR-33a showed attenuated colony formation capacity in TNBC cells. h–l TNBC cells were transfected with NC or EZH2 siRNA for 48 h and then cell cycle analysis was performed by a flow cytometer. j, k EZH2 siRNA decreased the number of migrated (j, k) and invasive (l) cells. Magnification, ×200. Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 compared with cells transfected with NC siRNA groups

Back to article page