Fig. 4

MiR-545-3p depletion overturns the effects of circ_0072083 knockdown in NSCLC cells treated with DDP. We transfected si-NC, si-circ_0072083, si-circ_0072083 + anti-miR-NC or si-circ_0072083 + anti-miR-545-3p into H522 and A549 cells. a, b The expression of miR-545-3p was detected in NSCLC cells by qRT-PCR. c, d The number of colonies was counted through colony formation assay in NSCLC cells treated with DDP. e, f The apoptosis of NSCLC cells treated with DDP was assessed by flow cytometry. g, h The expression of Bcl-2 and Bax was determined in NSCLC cells by western blot assay. i, j Flow cytometry was used to analyze the cell cycle of NSCLC cells treated with DDP. k, l Transwell migration assay was conducted to detect the migration of NSCLC cells treated with DDP. m, n The capacity of invasion in NSCLC cells treated with DDP was determined by transwell invasion assay. o, p Motility-associated proteins, such as E-cadherin, N-cadherin and Vimentin, were detected in NSCLC cells treated with DDP by western blot assay. *P < 0.05