Fig. 6

MiR-545-3p inhibits the malignant potential of NSCLC cells stimulated with DDP via inhibiting CBLL1. We co-transfected miR-NC, miR-545-3p, miR-545-3p + vector or miR-545-3p + CBLL1 into H522 and A549 cells. (a, b) The protein abundance of CBLL1 was measured in NSCLC cells by western blot assay. c, d The capacity of colony formation in NSCLC cells exposed to DDP was examined through colony formation assay. (e, f) The apoptosis of NSCLC cells stimulated with DDP was analyzed through flow cytometry. g, h Western blot assay was performed to measure the apoptosis-related proteins in NSCLC cells. i, j The cell cycle of NSCLC cells treated with DDP was analyzed using flow cytometry. k–n Transwell migration and invasion assays were applied to analyze the capacities of migration and invasion in NSCLC cells stimulated with DDP. o, p The protein levels of E-cadherin, N-cadherin and Vimentin were measured in NSCLC cells exposed to DDP by western blot assay. *P < 0.05