Table 1 Primer sequences for RT-qPCR
Genes | Sequence |
|---|---|
U6 | F: 5′-AAAGCAAATCATCGGACGACC-3′ |
R: 5′-GTACAACACATTGTTTCCTCGGA-3′ | |
GAPDH | F: 5′-TGTGGGCATCAATGGATTTGG-3′ |
R: 5′-ACACCATGTATTCCGGGTCAAT-3′ | |
ZEB1 | F: 5′-ACCTCTTCACAGGTTGCTCCT-3′ |
R: 5′-AGTGCAGGAGCTGAGAGTCA-3′ | |
Bcl-2 | F: 5′-GTCTTCGCTGCGGAGATCAT-3′ |
R: 5′-CATTCCGATATACGCTGGGAC-3′ | |
MDR1 | F: 5′-GCCTGGCAGCTGGAAGACAAATACACAAAATT-3′ |
R: 5′-CAGACAGCAGCTGACAGTCCAAGAACAGGACT-3′ | |
miR-129-5p | F: 5′-GATCCGCAAGCCCAGACCGCAAAAAGTTTTTA-3′ |
R: 5′-AGCTTAAAAACTTTTTGCGGTCTGGGCTTGCG-3′ | |
ZEB1-AS1 | F: 5′-GAGAGGCTAGAAGTTCCGCT-3′ |
R: 5′-ACAAGCACCGTGTGGGTATT-3′ |