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Fig. 2 | Cancer Cell International

Fig. 2

From: MiR-26a/miR-26b represses tongue squamous cell carcinoma progression by targeting PAK1

Fig. 2

Overexpressed miR-26a and miR-26b repressed TSCC cell cycle, migration and invasion. SCC4 and Cal27 cells were transfected with Mock (blank control), miR-NC, miR-26a mimic or miR-26b mimic, respectively. a, b RT-qPCR assay for the expression of miR-26a and miR-26b in transfected SCC4 and Cal27 cells, as determined by ANOVA analysis followed by Tukey test. c, d Flow cytometry assay for the cell cycle of transfected SCC4 and Cal27 cells, as determined by ANOVA analysis followed by Tukey test. e–h Transwell assay for the migrated and invasive abilities of transfected SCC4 and Cal27 cells, as determined by ANOVA analysis followed by Tukey test. i–j Western blot assay for the protein levels of MMP9, CCND1 and PCNA in transfected SCC4 and Cal27 cells, as determined by ANOVA analysis followed by Tukey test. **P < 0.01, ***P < 0.001

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