Fig. 4

Both miR-26a and miR-26b targeted PAK1. a, b Predicted binding sites between miR-26a/miR-26b and PAK1 together with the mutant sites in PAK1-MUT. c, d Dual-luciferase reporter assay for the luciferase activity of 293T cells co-transfected with PAK1-WT or PAK1-MUT luciferase reporter and miR-26a mimic, miR-26b mimic or miR-NC at 48 h after transfection, as determined by ANOVA analysis followed by Tukey test. e, f RIP and RT-qPCR assays for the PAK1 enrichment level in IgG or Ago2 immunoprecipitation complex of SCC4 and Cal27 cells transfected with miR-26a mimic, miR-26b mimic or miR-NC after 48 h, as determined by ANOVA analysis followed by Tukey test. ***P < 0.001