Fig. 6

Introduction of PAK1 partially reversed overexpressed miR-26a/miR-26b-mediated suppression of cell cycle, migration and invasion in TSCC cells. SCC4 and Cal27 cells were transfected with miR-NC, miR-26a/miR-26b mimic, miR-26a/miR-26b mimic + pcDNA Vector or miR-26a/miR-26b mimic + pcDNA-PAK1. a–d Flow cytometry assay for the cell cycle of treated SCC4 and Cal27 cells, as determined by ANOVA analysis followed by Tukey test. e–l Transwell assay for the migrated or invasive abilities of SCC4 and Cal27 cells, as determined by ANOVA analysis followed by Tukey test. m–p Western blot assay for the protein levels of MMP9, CCND1 and PCNA in transfected SCC4 and Cal27 cells, as determined by ANOVA analysis followed by Tukey test. *P < 0.05, **P < 0.01, ***P < 0.001