Fig. 4

RNA expression of drug efflux transporters, lysosomal proteins and cholesterol biosynthesis enzymes. RNA was purified from AML patient specimens using tri reagent, and gene expression levels were evaluated using quantitative RT-PCR, as described in the Methods section. Gene expression levels in relapsed patients (P1 and P2) at the different stages of their disease were compared to those in patients with good response to chemotherapy (GR1-5) at diagnosis. a–c Multidrug resistance efflux transporters, d Lysosomal proteins, and e Cholesterol biosynthesis enzymes. The results are displayed as fold over the internal control, GUSB