Fig. 6

RNA expression of de novo nucleotide biosynthesis pathway (DNSP) and folate metabolism genes. RNA was purified from AML patient specimens using tri reagent as described in the Methods section. Gene expression levels were evaluated using quantitative RT-PCR in relapsed patients (P1 and P2) at the different stages of their disease. The results are normalized to GUSB which served as an internal control, and presented as fold over P1-D