Fig. 5

TIMELESS decreased the acetylation of p65. a 293T cells were transfected with GFP-p65 only, GFP-p65 and Myc-CBP or GFP-p65, Myc-CBP and Flag-TIMELESS for 24 h. The extract was subjected to immunoprecipitation with anti-GFP antibody followed by western blot with anti-Myc or anti-Flag antibodies. b 293T cells were transfected with the vectors as described above. Cell lysate was immunoprecipitated with anti-GFP antibody followed by western blot with anti-acetylated lysine antibody. c T47D cells were transfected with GFP-p65 only, GFP-p65 and Myc-CBP together with or without shTIMELESS#1 for 24 h. The extract was subjected to immunoprecipitation with anti-GFP antibody followed by western blot with anti- acetylated lysine antibody