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Fig. 4 | Cancer Cell International

Fig. 4

From: CircTP63 promotes hepatocellular carcinoma progression by sponging miR-155-5p and upregulating ZBTB18

Fig. 4

CircTP63 serves as a miR-155-5p sponge in HCC cells. a The mRNA levels of U6 (nuclear control), GAPDH (cytoplasmic control), and circTP63 were measured using qRT‐PCR in SK-hep1 and SNU-387 cells. b A predicted binding site of miR-155-5p within circTP63 by bioinformatic analysis using the StarBase 3.0 (http://starbase.sysu.edu.cn/). Luciferase activity was determined in SK-hep1 and SNU-387 cells after transfection with miR-155-5p mimic or miRNA negative control (miR-NC). c, d RNA pull-down and luciferase reporter assays were conducted to determine the interaction between circTP63 and miR-155-5p. E. Relative mRNA expression of miR-155-5p in HCC tissues and adjacent normal tissues was evaluated by qRT-PCR. f Pearson correlation analysis between circTP63 and miR-155-5p expressions in 90 pairs of HCC tissues. Data are representative of three independent experiments and shown as mean ± SD., **P < 0.01; ns, no significance

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