Fig. 5

Effects of CDCA4 on EMT, migration and invasion could be influenced by autophagy activation. CDCA4 overexpressing and control A549 cells were treated with dimethyl sulfoxide (control) or rapamycin (100 nM) for 48 h. a Western blotting analysis was used to assess the expression of the indicated proteins in treated A549 cells. β-actin was used as a loading control. b Cell migration and invasion assays were performed using transwell compartments. CDCA4 knocking down and control H1299 cells were treated with dimethyl sulfoxide (control) or rapamycin (100 nM) for 48 h. c Western blotting analysis was used to assess the expression of the indicated proteins in treated H1299 cells. β-actin was used as a loading control. d Cell migration and invasion assays were performed using transwell compartments. Data are means ± S.D. *P < 0.05, **P < 0.01, ***P < 0.005