Fig. 6

Effects of CDCA4 on EMT, migration and invasion could be affected by autophagy inhibition. CDCA4 overexpressing and control A549 cells were treated with phosphate-buffered saline (control) or 3-methyladenine (5 mM) for 48 h. a Western blotting analysis was used to assess the expression of the indicated proteins in treated A549 cells. β-actin was used as a loading control. b Cell migration and invasion assays were performed using transwell compartments. c,d. CDCA4 knocking down and control H1299 cells were treated with phosphate-buffered saline (control) or 3-methyladenine (5 mM) for 48 h. c Western blotting analysis was used to assess the expression of the indicated proteins in treated H1299 cells. β-actin was used as a loading control. d Cell migration and invasion assays were performed using transwell compartments. Data are means ± S.D. *P < 0.05, **P < 0.01, ***P < 0.005