Fig. 7

CDCA4 interacts with CARM1 and suppresses CARM1 expression. a CO-IP assays were performed in A549 CDCA4 overexpressing cells. b Western blotting. analysis was used to assess the expression of CARM1 and CDCA4 in CDCA4 overexpressing cells or knocking down cells. β-actin was used as a loading control. c Western blotting analysis and qPCR analysis of efficiency of knocking down CDCA4 in both A549 and H1299 cells. d Western blotting analysis of P62 and LC3B protein expression. β-actin was used as a loading control. e Cell migration and invasion assays were performed using transwell compartments. Data are means ± S.D. *P < 0.05, **P < 0.01, ***P < 0.005