Fig. 8

CARM1 knockdown could alter the functions of CDCA4 both in inducing autophagy and suppressing migration. b. CDCA4 overexpressing and control A549 cells were transfected with siCARM1. a Western blotting analysis was used to assess the expression of the indicated proteins in treated A549 cells. β-actin was used as a loading control. b Cell migration and invasion assays were performed using transwell compartments. c.d. CDCA4 knocking down and control H1299 cells were transfected with siCARM1. c Western blotting analysis was used to assess the expression of the indicated proteins in treated A549 cells. β-actin was used as a loading control. d Cell migration and invasion assays were performed using transwell compartments. e qPCR analysis of seven autophagy-related genes. Data are means ± S.D. *P < 0.05, **P < 0.01, ***P < 0.005