Exosome-based detection of EGFR T790M in plasma and pleural fluid of prospectively enrolled non-small cell lung cancer patients after first-line tyrosine kinase inhibitor therapy

Table 4 Performance comparison for the detection of the EGFR T790M mutation between exoTNA from supernatant and total DNA from cell pellets isolated from pleural fluid (n = 13)

EGFR genotype	TP and TN^a	ddPCR using exoTNA extracted from supernatants		ddPCR using total DNA extracted from cell pellet
EGFR genotype	TP and TN^a	Mutant type	Wild-type	Mutant type	Wild-type
Mutant type	7	7	0	5	2
Wild-type	6	0	6	0	6
Sensitivity,% (95% CI)		100 (97.3–103)		71.4 (68.7–74.1)
Specificity, % (95% CI)		100 (97.3–103)		100 (97.3–103)
Accuracy, % (95% CI)		100 (97.3–103)		84.6 (81.9–87.3)

^a‘True positive’ was defined by a positive T790M mutation in one or more liquid biopsy platforms among exoTNA from supernatants and cellular total DNA from pleural fluid; ‘True negative’ was defined as T790M mutation negative in all tested liquid biopsy platforms
TP true positive, TN true negative, CI confidence interval

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