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Fig. 2 | Cancer Cell International

Fig. 2

From: Three novel circRNAs upregulated in tissue and plasma from hepatocellular carcinoma patients and their regulatory network

Fig. 2

Identification of three up-regulated circRNAs in HCC tissues and plasma samples. a, b PCA plot of the first two components before (left) and after (right) Limma standardization, which minimizes the batch effect. c Heatmap for ten selected differentially expressed circRNAs in the pooled 15 pairs of HCC samples. d Venn diagram of up-regulated and down-regulated circRNAs in the HCC tissues-related meta-cohort (GSE78520&GSE94508&GSE97332) and the HCC plasma samples-related microarray dataset (GSE135806). e Volcano plot showing three up-regulated circRNAs in HCC based on datasets GSE78520&GSE94508&GSE97332 and GSE135806, respectively. f Expression of the three identified circRNAs in the respective datasets. CHB, chronic hepatitis B patients. Data were shown as mean ± SEM. g Agarose gel electrophoresis showed that divergent primers amplified hsa_circ_0009910 (left) and hsa_circ_0089172 (right) in cDNA but not genomic DNA (gDNA) from HepG2 cells. h Real-time PCR (top) and agarose gel electrophoresis (bottom) showed that hsa_circ_0009910 and hsa_circ_0089172 but not β-actin from HepG2 cells were insensitive to RNase R treatment. i Detection of hsa_circ_0009910 (left) and hsa_circ_0089172 (right) excretion in the indicated hepatoma cells’ culture medium

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