Fig. 2

Hsa_circ_0010235 knockdown inhibited proliferation and autophagy but facilitated apoptosis in NSCLC cells. a QRT-PCR assay for the relative expression of hsa_circ_0010235 in H1299 and A549 cells transfected with si-NC, si-hsa_circ_0010235#1 or si-hsa_circ_0010235#2. b QRT-PCR assay for the relative expression of hsa_circ_0010235 in H1299 and A549 cells transfected with pCD-ciR or hsa_circ_0010235. c–j H1299 and A549 cells were transfected with si-NC, si-hsa_circ_0010235#1, pCD-ciR or hsa_circ_0010235. c, d CCK-8 assay for the cell viability of transfected cells. e Colony formation assay for the colony formation ability of transfected cells. f Flow cytometry for the apoptotic rate of transfected cells. g, h Flow cytometry for the cell cycle distribution in G0/G1, S and G2/M phases of transfected cells. i, j Western blot assay for the protein levels of LC3-I, LC3-II and p62 in transfected cells. *P < 0.05