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Fig. 7 | Cancer Cell International

Fig. 7

From: Hsa_circ_0010235 functions as an oncogenic drive in non-small cell lung cancer by modulating miR-433-3p/TIPRL axis

Fig. 7

Silencing of miR-433-3p or overexpression of TIPRL could attenuate hsa_circ_0010235 knockdown-induced inhibitory effects on NSCLC progression. H1299 and A549 cells were transfected with si-NC, si-hsa_circ_0010235#1, si-hsa_circ_0010235#1 + anti-miR-433-3p or hsa_circ_0010235#1 + TIPRL. a QRT-PCR assay for the relative mRNA expression of TIPRL in transfected cells. b, c Western blot assay for the protein level of TIPRL in transfected cells. d, e CCK-8 assay for the cell viability of transfected cells. f Colony formation assay for the colony formation ability of transfected cells. g Flow cytometry for the apoptotic rate of transfected cells. h, i Flow cytometry for the cell cycle distribution in G0/G1, S and G2/M phases of transfected cells. j, k Western blot assay for the protein levels of LC3-I, LC3-II and p62 in transfected cells. l, m Transwell assay for the migration and invasion of transfected cells. n Wound healing assay for the migration capacity of transfected cells. o Spearman’s correlation analysis for the expression levels of hsa_circ_0010235 and TIPRL mRNA in 52 NSCLC tissues. *P < 0.05

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