Fig. 2
From: Postponing tumor onset and tumor progression can be achieved by alteration of local tumor immunity
Mammary tumor growth and lung metastasis are suppressed by a B6 genetic background. a Total tumor burdens were determined in Fvb.B6 (n = 10) and Fvb (n = 10) mice by dissecting and weighing all tumors at 10, 12 and 14 weeks old. b The appearance of mammary tumors was monitored in Fvb.B6 (n = 30) and Fvb (n = 30) mice by weekly palpation and is represented on a Kaplan–Meier curve. c Ki67 staining of the mammary tumor sections from adenoma and late carcinoma. d. The mean percentage of Ki67 positive cells ± SEM in tumors from 5 mice/genotypes. e, f Bouin’s fixed lungs from Fvb.B6 and Fvb e mice were stained with Bouin’s solution to identify metastases (arrows). The data represents the mean total number of metastases ± SEM from Fvb.B6 (n = 9) and Fvb (n = 12) mice at 14 weeks old (f). g, h Formalin-fixed, paraffin-embedded lung sections from Fvb.B6 and Fvb g mice were stained with hematoxylin and eosin (H&E) to identify metastases (arrows). The data represents the mean total number of metastases from five lung sections/mouse ± SEM from Fvb.B6 (n = 8) and Fvb (n = 9) mice (h). Data represents the mean ± SEM, NS = non-significant, *P < 0.05, **P < 0.01, and ***P < 0.001