Fig. 2

Circ_0000020 was involved in promoting the proliferation, migration and invasion of glioma cells. a Empty vector, circ_0000020 overexpression plasmid, si-NC and si-circ_0000020 were transfected into U87 and U251 cell line. The expression of circ_0000020 was detected by qRT-PCR 48 h after the transfection. (n = 3). b 48 h after the transfection, CCK-8 assay was used to detect cell proliferation after knockdown or overexpression of circ_0000020. (n = 3). c Transwell assay was used to detect cell migration and invasion after the knockdown or overexpression of circ_0000020 (magnification: ×400). (n = 3). si-NC: siRNA normal control, si-circ_0000020: siRNA against circ_0000020, Vector: pcDNA empty vector, circ_0000020: pcDNA-circ_0000020, Error bars represented the mean ± SD of at least three independent experiments; *P < 0.05, **P < 0.01 and ***P < 0.001