Fig. 3

Circ_0000020 targeted miR-142-5p in glioma. a The potential targets of circ_0000020 were predicted by bioinformatics analysis, and circ_0000020 contained a potential binding site of miR-142-5p. b The expression of miR-142-5p in glioma tissues and normal tissue adjacent to tumor was detected by qRT-PCR. c qRT-PCR was used to detect the expression of miR-142-5p in NHA and 5 kinds of glioma cells (U87, SW1008, T98G, U251 and U138). (n = 3). d Person’s correlation coefficient was used to analyze the correlation between circ_0000020 and miR-142-5p expressions in glioma tissues. e WT-circ_0000020 reporter, Mut-circ_0000020 reporter, together with miR-142-5p mimics or miR NC, were co-transfected into U87 and U251 cells, respectively, and dual-luciferase gene reporter assay was used to validate the predicted binding site between circ_0000020 and miR-142-5p. (n = 3). f qRT-PCR was used to detect miR-142-5p expression in glioma cells after knockdown or overexpression of circ_0000020. (n = 3). N: normal tissue; T: tumor tissue; Control: pmirGLO-control, WT-circ_0000020: WT-pmirGLO-circ_0000020, Mut-circ_0000020: Mut-pmirGLO-circ_0000020, miR-142-5p: miR-142-5p mimics, miR NC: miR negative control, Error bars represented the mean ± SD of at least three independent experiments; **P < 0.01, ***P < 0.001, and NS: P > 0.05