Fig. 3
From: LncRNA ACTA2-AS1 suppress colon adenocarcinoma progression by sponging miR-4428 upregulation BCL2L11
LncRNA ACTA2-AS1 sponged miR-4428 in COAD cells. a LncRNA ACTA2-AS1 was predicated to function as molecular sponge for miR-4428 using an online database lncBASE. b The putative binding sites between ACTA2-AS1 and miR-4428. Relative miR-4428 expression in cells co-transfected with wt or mut ACTA2-AS1 and miR-4428 in SW480 and HT29 cells by luciferase reporter assay. c Enrichment of lncRNA ACTA2-AS1 using RNA pull-down experiments in SW480 and HT29 cells. d Relative miR-4428 expression in SW480 and HT29 cells transfected with sh-NC, sh-ACTA2-AS1, empty vector and OE ACTA2-AS1. e Relative miR-4428 expression in 82 pairs of COAD tissues and their adjacent normal tissues using qRT-PCR assay. f Spearman’s rank-order correlation between miR-4428 and ACTA2-AS1. *p < 0.05; **p < 0.01