Skip to main content
Fig. 5 | Cancer Cell International

Fig. 5

From: LncRNA ACTA2-AS1 suppress colon adenocarcinoma progression by sponging miR-4428 upregulation BCL2L11

Fig. 5

LncRNA ACTA2-AS1 suppressed COAD progression by sponging miR-4428 upregulation BCL2L11. a Relative BCL2L11 mRNA expression in HT29 cells transfected with sh-NC and sh- ACTA2-AS1. b Relative BCL2L11 mRNA expression in SW480 transfected with sh-NC, sh-ACTA2-AS1, sh-ACTA2-AS1 + miR-4428 inhibitor and sh-ACTA2-AS1 + BCL2L11. And relative BCL2L11 mRNA expression in HT29 cells transfected with OE ACTA2-AS1, OE ACTA2-AS1 + miR-4428 mimics, and OE ACTA2-AS1 + si-BCL2L11, respectively. c Relative BCL2L11 mRNA protein in SW480 transfected with sh-NC, sh-ACTA2-AS1, sh-ACTA2-AS1 + miR-4428 inhibitor and sh-ACTA2-AS1 + BCL2L11. And relative BCL2L11 protein expression in HT29 cells transfected with OE ACTA2-AS1, OE ACTA2-AS1 + miR-4428 mimics, and OE ACTA2-AS1 + si-BCL2L11, respectively. d Apoptosis-related protein Bax and Bcl-2 expression in SW480 and HT29 cells transfected with sh- ACTA2-AS1, sh-ACTA2-AS1 + miR-4428 inhibitor and sh-ACTA2-AS1 + BCL2L11, and OE ACTA2-AS1, OE ACTA2-AS1 + miR-4428 mimics, and OE ACTA2-AS1 + si-BCL2L11, respectively. e CCK-8 assay of cell viability in SW480 and HT29 cells transfected with sh- ACTA2-AS1, sh-ACTA2-AS1 + miR-4428 inhibitor and sh-ACTA2-AS1 + BCL2L11, and OE ACTA2-AS1, OE ACTA2-AS1 + miR-4428 mimics, and OE ACTA2-AS1 + si-BCL2L11. f Colony formation in SW480 and HT29 cells transfected with sh- ACTA2-AS1, sh-ACTA2-AS1 + miR-4428 inhibitor and sh-ACTA2-AS1 + BCL2L11, and OE ACTA2-AS1, OE ACTA2-AS1 + miR-4428 mimics, and OE ACTA2-AS1 + si-BCL2L11. g Flow cytometric analysis showing the effects of the cell apoptosis in SW480 and HT29 cells transfected with sh-ACTA2-AS1, sh-ACTA2-AS1 + miR-4428 inhibitor and sh-ACTA2-AS1 + BCL2L11, and OE ACTA2-AS1, OE ACTA2-AS1 + miR-4428 mimics, and OE ACTA2-AS1 + si-BCL2L11, respectively. **p < 0.01

Back to article page