Fig. 6

CFL-1, LIMK1 and SSH1 expression, correlation and prognostic value according to the CMS classification using RNA-Seq data from TCGA Data Bank. a Tumor tissues were grouped according to the CMS classification (n = 468), and CFL-1, LIMK1, and SSH1 mRNA levels in each subtype were plotted. Kruskal Wallis followed by Dunn’s test were used to compare rank means between each subgroup. Asterisks indicate the padj: *P < 0.05; **P < 0.01; ***P < 0.001 and ****P < 0.0001. Not significant (n.s.). b Correlation between CFL-1 and SSH1 or c LIMK1 mRNA levels according to CMS classification. The P values and R coefficients were derived from the Spearman correlation test. d Kaplan–Meier curves depicting the overall survival (5 years) of CRC patients stratified based on the LIMK1 expression levels according to CMS2 canonical (high n = 113; low n = 112), and CMS3 metabolic subtypes (high n = 36; low n = 36). Samples expressing the gene above or equal the median were classified as high expression and the others as low expression. The P values were derived from the log-rank test, and are indicated. CMS1 immune, CMS2 canonical, CMS3 metabolic, and CMS4 mesenchymal