Fig. 2
From: Cisplatin loaded multiwalled carbon nanotubes reverse drug resistance in NSCLC by inhibiting EMT
Pt-MWNTS reverse drug resistance and affect colony formation of A549/DDP. MTT assay of NSCLC cell lines a A549 and b A549/DDP treated by Pt and Pt-MWNTS at different cisplatin concentration for 48 h (n = 5). c Using different concentration of MWNTS to treat A549 and A549/DDP cells for 48 h (n = 5 per group). d–f Colony formation assay of A549 and A549/DDP cells. Cells were seed in 10-cm dish at a density of 500 cells/dish. Then cells were treated with PBS (control), MWNTS, cisplatin (Pt) and Pt-MWNTS at a concentration of 50 μM. After 7 days, we stained the dishes with crystal violet to count the number of colonies. The colony formation rate was calculated by divide the total colony number in each well by 500. (n = 3, **P < 0.01, ***P < 0.001 vs Control; ##P < 0.01 vs Pt.)