Fig. 2

ROC1 regulation of tumor cell growth through hedgehog signaling. a, b Western blot. Stable ROC1-overexpressed and transient ROC1 siRNA-transfected 5637 (a) and T24 (b) cells were grown and subjected to western blot analysis of cyclin D1 and Cdc25c expression. c, d qRT-PCR. Stable ROC1-overexpressed and transient ROC1 siRNA-transfected 5637 and T24 cells were grown and subjected to qRT-PCR analysis of Gli1 and PTCH1. e, f Western blot. Transient ROC1 siRNA-transfected 5637 cells were treated with SAG (e), stable ROC1-overexpressed T24 cells were treated with the hedgehog signaling pathway inhibitor GDC0449 (f), and then the cells were subjected to western blot analysis of Gli1 and Gli2. Bars, SEM; *P < 0.05, **P < 0.01, ***P < 0.001