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Fig. 4 | Cancer Cell International

Fig. 4

From: Regulator of cullins-1 (ROC1) negatively regulates the Gli2 regulator SUFU to activate the hedgehog pathway in bladder cancer

Fig. 4

ROC1 regulation of SUFU ubiquitination for degradation. a, b Western blot. ROC1-knocked down and ROC1-overexpressed 5637 (a) and T24 (b) cells were treated with cycloheximide (CHX) for the indicated time points and then subjected to western blot analysis of SUFU protein. The graph shows the quantified data of the western blots depicted in the bottom panel. c, d Coimmunoprecipitation. Detection of ubiquitylated SUFU in 5637 (c) and T24 (d) cells cotransfected with HA-tagged ubiquitin (Ub) along with either siROC1 or pROC1 by western blot analysis of the cell lysates. Immunoprecipitation of HA antibody of nonspecific rabbit immunoglobulin G (IgG) was used as a negative control. e, f Western blot. 5637 (e) and T24 (f) cells were treated with the CRL inhibitor MLN4924 at different concentrations and then subjected to western blot analysis of Gli2, cyclin D1, Cdc25c, and SUFU proteins

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Cancer Cell International

ISSN: 1475-2867

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