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Fig. 3 | Cancer Cell International

Fig. 3

From: The lncRNA NEAT1 promotes the epithelial-mesenchymal transition and metastasis of osteosarcoma cells by sponging miR-483 to upregulate STAT3 expression

Fig. 3

miR-483 inhibits the EMT in osteosarcoma cells by suppressing STAT3 expression.U2OS cells were transfected with mimics NC, miR-483 mimics, empty vector (OE-NC), a STAT3 overexpression plasmid (OE-STAT3), or miR-483 mimics plus the STAT3 overexpression plasmid. ac, e The migration of U2OS cells was measured using the scratch wound healing experiment (a, b) or transwell migration experiment (c, e). Scale bar: 200 μm. d, e The invasion of U2OS cells was determined using a transwell invasion assay. Scale bar: 200 μm. f The relative expression of STAT3, E-cadherin, N-cadherin, Vimentin, and Snail in U2OS cells was determined using qRT-PCR. The mRNA levels were normalized to the GAPDH mRNA. g The phosphorylation of STAT3 and the levels of STAT3, STAT1, E-cadherin, N-cadherin, Vimentin, and Snail in U2OS cells were examined using western blotting. GAPDH was employed as the input control. All experiments described in this study were performed at least three times, which referred to biological replicates. Each biological replicate consisted of 3 wells of cells at the same passage, which referred to technical replicates. Error bars denoted mean ± SD. p values were determined using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. ***p < 0.001, **p < 0.01, and *p < 0.05

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