Fig. 2

Knockdown of PR55α expression induces cell proliferation and increases cell migration and invasion. a PR55α expressions across one normal liver cell line and five lines of liver cancer cells as demonstrated using western blot. siRNA efficacy in knocking down PR55α as tested by western blot (b) and real-time PCR (c). d Migration (above) and invasion (down) assays in SMMC-7721 and LM3 lines (200× magnification). e Colony formation assays for liver cancer cells transfected with shPR55α. f Growth curves in PR55α control and knockdown cells. Each experiment was an average of results obtained from three replicates and is depicted in terms of mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001