Fig. 1

Let-7a and miR-1182 may affect CCA progression via regulating NUAK1. a Heat map of DEGs in GSE45001 microarray (the abscissa indicates the sample number, the ordinate indicates the gene name, the upper dendrogram indicates the sample type cluster, and the left dendrogram indicates gene expression cluster, each small square indicates the expression of one gene in one sample, and the histogram in the right upper is color gradation). b The expression of NUAK1 gene in the CCA expression microarray GSE89749 (the left box plot represents the expression of NUAK1 in the normal sample, and the right box plot represents the expression of NUAK1 in the CCA sample). c NUAK1 gene expression in the TCGA-listed CCA database (the left box plot represents the expression of NUAK1 in the normal sample and the right box plot represents the expression of NUAK1 in the CCA sample). d Correlation analysis of NUAK1 and CCA-related genes (each small circle in the Fig. represents one gene; the darker the color, the more central the position of the gene in the network, the line between two genes indicates there is an interaction between them). e The prediction result of miRNAs targeting NUAK1 gene, (the left side is the first 80 miRNAs predicted by the RNA22 database, the right side is the first 80 miRNAs predicted by the miRGator database, and the middle part indicates the intersection of the two databases). CCA cholangiocarcinoma, NUAK1 novel (nua) kinase family 1, DEGs differentially expressed genes, TCGA The Cancer Genome Atlas, CHOL cholesterol