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Fig. 6 | Cancer Cell International

Fig. 6

From: MicroRNA-1182 and let-7a exert synergistic inhibition on invasion, migration and autophagy of cholangiocarcinoma cells through down-regulation of NUAK1

Fig. 6

Up-regulated miR-1182 and let-7a induces cell autophagy. a MDC staining results (×400). b Statistical results of cell autophagy rate in each group. c The mRNA expression of Beclin1 and LC3 in HCCC-9810 and Huh28 cells measured by RT-qPCR. d The protein bands of Beclin1, LC3I, and LC3II determined by Western blot analysis. e Statistical results of the Beclin1, LC3I, and LC3II protein levels determined by Western blot analysis. * p < 0.05 vs. that of cells transfected with miR-NC or pc-DNA, #p < 0.05 vs. that of cells transfected with miR-1182 mimic or let-7a mimic, and & p < 0.05 vs. that of cells transfected miR-1182 mimic + let-7a mimic. The statistical data were measurement data and expressed as mean ± standard deviation. Comparisons among multiple groups were analyzed using ANOVA and followed by Tukey’s post hoc test. The experiment was repeated 3 times independently

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