|
Gene
|
Sequence (5′-3′)
|
|---|
|
miR-1182
|
F: GGGGAGGGTCTTGGGAGGGA
|
|
R: GTGCAGGGTCCGAGGT
|
|
miR-let-7a
|
F: TGAGGTAGTAGGTTGTGTGGTT
|
|
R: GTGCAGGGTCCGAGGT
|
|
NUAK1
|
F: GAAGTTATGCTTTATTCAC
|
|
R: CATCCTCTGATTCTAGGTG
|
|
MMP-2
|
F: AGTTTCCATTCCGCTTCCAG
|
|
R: CGGTGGTAGTCCTCAGTGGT
|
|
MMP-9
|
F: ACTACTGTGCCTTTGAGTCC
|
|
R: AGAATCGCCAGTACTTCCCA
|
|
Beclin1
|
F: ATGCAGGTGAGCTTCGTGTG
|
|
R: CTGGGCTGTGCTAAGTAATGCA
|
|
LC3
|
F; AATCCCGGTGATCATCGAGC
|
|
R: GCCGGATGATCTTGACCAAC
|
|
U6
|
F: ATTGGAACGATACAGAGAAGATT
|
|
R: GGAACGCTTCACGAATTTG
|
|
GAPDH
|
F: GGGAAACTGTGGCGTGAT
|
|
R: GAGTGGGTGTCGCTGTTGA
|
- RT-qPCR reverse transcription quantitative polymerase chain reaction, MiR-1182 microRNA-1182, miR-let-7a microRNA-let-7a, NUAK1 Novel (nua) kinase family 1, MMP-2 matrix metalloproteinase-2, MMP-9 matrix metalloproteinase-9, LC3 light chain 3, GAPDH glyceraldehyde-3-phosphate dehydrogenase
