Fig. 5

Gedatolisib increased the expression level of ABCB1 or ABCG2 in drug resistance cells without altering the subcellular localization of ABCB1 or ABCG2. a Protein expression level of ABCB1 in SW620/ADR cells after treatment with gedatolisib (1 μM) for 24, 48, and 72 h. b Protein expression level of ABCG2 in LS180/MX cells after treatment with gedatolisib (1 μM) for 24, 48, and 72 h. Relative expression level of ABCB1 (c) and ABCG2 (d) after treatment with 1 μM of gedatolisib. e Subcellular localization of ABCB1 in SW620/ADR cells after treatment with gedatolisib (1 μM) for 72 h. f Subcellular localization of ABCG2 in LS180/MX cells after treatment with gedatolisib (1 μM) for 72 h. Green represents ABCB1 or ABCG2, red shows the nuclei which is dyed by PI. Scale bar 50 μm. Data were shown as mean ± SD, which is representative for three independent experiments. Asterisk presents when p value is < 0.05