Fig. 4

Effects of Knockout of CALM1 and application of EGFR inhibitor on tumorigenesis in nude mice in vivo. KYSE150 cells that were infected with CALM1 or scramble lentivirus were injected s.c. into nude mice. a-c Nude mice harboring subcutaneous tumors derived from implanted Control CALM1 and CALM1 KO cells were treated by subcutaneous injection with vehicle (saline) or Afatinib. Tumor dimensions were recorded on every other day and calculated tumor volumes are presented as the mean ± SD for each group (n = 10). Weight of terminal tumors was recorded on Day 27. d, e The total radiant efficiency of the ROI in xenografts from mice injected with Control CALM1 and CALM1 KO cells with vehicle (saline) or Afatinib. CALM1, calmodulin1; EGFR, epidermal growth factor receptor. ESCC, esophageal squamous cell carcinoma; ROI Region of Interest; *P < 0.05, **p < 0.01, ***p < 0.001 (Student's t-test). F, Immunostaining for Cleaved caspase-3 and Ki-67 on tumor lesions dissected from nude mice in the three groups. KO, knock-out of CALM1. Scale bar, 100 μm. Shown was the representative figures selected among candidates. G, Schematic diagram of the findings we described in our paper