Fig. 2

Effect of LXR agonist (T0901317) on the ABC transporter gene expression and protein levels LXRs in CSC. a mRNA expression levels of ABCA1, ABCG5, and ABCG8 levels were determined by RT-PCR after incubation of the treatment group with 5 µM T0901317 for 48 h. b Protein levels of ABCA1, ABCG5, ABCG8, and LXRs were determined by western blotting. CSCs were treated with 5 µM T0901317 for 48 h. Measuring CSCs resistance to hypo-osmotic stress after treatment with LXR agonist. c Changes in diameter of CSCs were recorded after exposure to 5 µM T0901317 every 15 min over 45 min (n = 60). d CSC diameter was increased in treated groups compared to the control group. All experiments were performed in triplicate and data were expressed as mean ± SD. Tukey posthoc analysis. *p < 0.05, **p < 0.01, and ***p < 0.001