Fig. 4

Effect of SR9243 on metabolic LXR-targets genes in CSCs. a mRNA expression levels of PFKFB3, GSK3β, SCD1, FASN, and HIF-1α were determined by real-time PCR after incubation with 60 nM SR9243 over 48 h. b Protein levels of PFKFB3, GSK3β, SCD1, FASN, HIF-1α, and LXRs were determined by western blotting in CSCs after incubation with 60 nM SR9243. All experiments were performed in triplicate and data were expressed as mean ± SD. Student t-test. *p < 0.05, **p < 0.01, and ***p < 0.001