Fig. 3
RNF152 acts as a tumor suppressor in HCC. a HCC cells with or without RNF152 overexpression were subjected to BrdU test. ***P < 0.001. EV: empty vector. b HCC cells with or without RNF152 overexpression were examined for colony formation. *** P < 0.001. c HCC cells with or without RNF152 overexpression were examined for cell invasion. ***P < 0.001, **P < 0.01. d HCC cells with or without RNF152 silencing (RNF152-shRNA1-3) were examined for FBXO16 mRNA expression by real-time PCR. ***P < 0.001. e RNF152-depleted HuH6 cells were transfected with a shRNA-resistant RNF152 plasmid. The RNF152 mRNA levels in these cells were examined by real-time PCR. ***P < 0.001. f HCC cells in e were subjected to BrdU test. ***P < 0.001. g HCC cells in e were examined for colony formation. ***P < 0.001. h HCC cells in e were examined for cell invasion. ***P < 0.001. i Each nude mouse was subcutaneously injected with 1 × 107 control HuH6 cells or RNF152-depleted HuH6 cells, and continued observation for 4 weeks. Tumour growth was measured using a caliper at the indicated times after injection. n = 7 for each group. ***P < 0.001. j Mice were sacrificed 4 weeks after HCC cells injection. The tumors were then excised, washed with PBS and photographed. K Tumor weights were measured after mice were sacrificed. ***P < 0.001. l RNF152 mRNA expression levels in tumors from each group were examined by real-time PCR. ***P < 0.001