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Fig. 4 | Cancer Cell International

Fig. 4

From: LINC00958 promotes the proliferation of TSCC via miR-211-5p/CENPK axis and activating the JAK/STAT3 signaling pathway

Fig. 4

LINC00958 acts as a ceRNA via binding to miR-211-5p. a LINC00958 was predicted to be located mainly in the cytoplasm using the bioinformatics tools in lncATLAS. b RT-qPCR analysis of subcellular LINC00958 expression in the nucleus and cytoplasm of CAL-27 and SCC-9 cells. GAPDH, β-actin and U6 were used as endogenous controls. c FISH assay was performed to detect the subcellular localization of LINC00958 in CAL-27 and SCC-9 cells. LINC00958 is stained green and nuclei are stained blue (DAPI). d The predicted miR-211-5p binding sites in the LINC00958 transcript. e Luciferase activities in CAL-27 cells co-transfected wild-type (WT) or mutant LINC00958 plasmid together with miR-211-5p mimic or miR-Ctrl. f Enrichment of LINC00958 and miR-211-5p in the Ago2 immunoprecipitation compared with the control IgG precipitation. g Correlation between LINC00958 and miR-211-5p expression in TSCC tissues based on the TCGA database. h RT-qPCR of miR-211-5p in CAL-27 and SCC-9 cells stably transfected with sh-LINC00958, pcDNA-LINC00958 and their corresponding control vectors. i RT-qPCR of LINC00958 in CAL-27 and SCC-9 cells transfected with miR-211-5p mimics, miR-211-5p inhibitor and their corresponding controls *P < 0.05, **P < 0.01, ***P < 0.001

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