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Fig. 4 | Cancer Cell International

Fig. 4

From: CircMED13L_012 promotes lung adenocarcinoma progression by upregulation of MAPK8 mediated by miR-433-3p

Fig. 4

circMED13L_012 modulates miR-433-3p expression. a Bioinformatic prediction of circMED13L_012 interacting miRNAs using Circinteractome and Starbase online database platform. bd Association of circMED13L_012 expression level and miR-433-3p, miR-503-3p, miR-330-3p expression level in NSCLC tumor samples. e Design of wildtype and mutated circMED13L_012 vector and predicted binding location with miR-433-3p. f, g Dual luciferase reporter gene assay via wildtype or mutated circMED13L_012 vector transfection with or without treatment of sh-circMED13L_012 to validate the binding of miR-433-3p with wildtype circMED13L_012. h, i. RNA immunoprecipitation assay to detect the binding of circMED13L_012 and miR-433-3p with anti-Ago2 antibody, in comparison with control IgG antibody in A549 and NCI-H1299 tumor cells. j. qRT-PCR evaluation of the impact of circMED13L_012 overexpression and silencing on expression level of miR-433-3p in NCI-H1299 and A549 cells.

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